The expected molecular weight of the protein is 236kDa. I have the commercially purchased cDNA clone of the same in a Myc-DDK backbone under a CMV promoter The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is a multifunctional, nuclear phosphoprotein that functions as a transcription factor with a theoretical molecular weight of 62kDa MYC15-R | Recombinant purified Myc-tag -Protein (~9 kda) control for ELISA size: 100 ug | 393.18 US
Size 1 mg. Purity >96% (HPLC) Molecular Weight 2.4 kDa. Reconstitution Reconstitute 2xMyc-peptide in phosphate buffered saline (PBS) pH 7.4 to a final concentration of 1 mg/ml. Storage instructions Shipped at ambient temperature. Upon receipt store at -20°C. Aliquot upon reconstitution and store at -20°C. Avoid repeated freeze/ thaw cycles The Myc epitope tag contains 10 amino acids, and has a molecular weight of 1.2 KDa. Anti-Myc Tag antibodies facilitate the detection, isolation, and purification of Myc-tagged target proteins without a protein-specific antibody or probe Human c-Myc protein: 10 Amino acids (1.2 kDa) (EQKLISEEDL) c-Myc protein is a transcription factor involved in the cell cycle and apoptosis. Abnormalities in c-Myc, such as mutations and overexpression, are associated with various cancers of the hematopoietic system. : V5: Virus: 14 Amino acids (1.4 kDa) (GKPIPNPLLGLDST biomapping Other tags with cost-effective purification resins are Maltose-Binding Protein (MBP), Cellulose-Binding Domain (CBD), and Glutathione S-Transferase (GST) . These tags are approximately 40 times the size of metal affinity tags, so they may interfere with the structure or function of the fusion partner
Plasmid pcDNA3 mHIF-2α MYC (P405A/P530V/N851A) from Dr. Celeste Simon's lab contains the insert mHIF-2α (P405A/P530V/N851A) and is published in Mol Biol Cell. 2007 Nov;18(11):4528-42. Epub 2007 Sep 5. This plasmid is available through Addgene 05-724. Sigma-Aldrich. Anti-Myc Tag Antibody, clone 4A6. Download. Zoom. This Anti-Myc Tag Antibody, clone 4A6 is validated for use in ChIP, IC, IF, IP, WB for the detection of Myc Tag. This antibody has been cited in more than 25 published papers Plasmid myc-BioID2-MCS from Dr. Kyle Roux's lab is published in Mol Biol Cell. 2016 Feb 24. pii: mbc.E15-12-0844. This plasmid is available through Addgene With 6 His, the protein will have an added 1 kDa of molecular weight. Often, a linker (such as gly-gly-gly or gly-ser-gly) is placed between the protein of interest and the 6 His tag in order to prevent the polyhistidine tag from affecting the activity of the protein being tagged
Myc-DDK-tagged ORF clone of viral ORF for 445 kDa protein [Human adenovirus 54], codon optimized for human cell expression, YP_00303862 Myc-tag (Kd: 400 nM) and to multiple Myc-tag repeats (Kd: 0.5 nM). Binding capacity: 17.5 µg of recombinant Myc-tagged protein (~42 kDa) per 25 µL bead slurry Bead size: 90 µm (cross-linked 4 % agarose beads Anti-MYC tag mouse monoclonal antibody,at 1:100 dilution . Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 39 kDa. Observed band size: 39 kDa A ~17 kDa band corresponding to HA tagged Histone H3 was observed in the transfected samples. Known quantity of protein samples were electrophoresed using NuPAGE 10 % Bis-Tris, 1.0 mm, Mini Protein Gel, 12-well (Cat. No. NP0302BOX) , XCell SureLock Electrophoresis System (Cat. No. EI0002) , and Novex Sharp Pre-Stained Protein Standard (Cat. No. LC5800)
방문 중인 사이트에서 설명을 제공하지 않습니다 These results indicated that the maximal size allowed to diffuse through the nuclear pore is between that of Myc-ERK2x2+100 aa and Myc-ERK2x2+200 aa (98 and 110 kDa, respectively). Lastly, as another confirmation that nuclear pore is able to allow the diffusion of proteins with a size of ∼90 kDa, serial artificial proteins based on 3′-phosphoinositide-dependent kinase 1 (PDK1) were made Anti-Myc Tag Antibody Upstate®, from rabbit; Synonyms: Transcription factor p64, avian myelocytomatosis viral oncogene homolog, myc proto-oncogene protein, v-myc avian myelocytomatosis viral oncogene homolog, v-myc myelocytomatosis viral oncogene homolog (avian); find Sigma-Aldrich-06549 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldric The GST-tag has been in use for protein purification since the late 1980s (Smith & Johnson 1988) and has been established as a reliable way for protein purification assays that required highest level of purity (Harper & Speicher 2013). The size of GST-tag is 26 kDa and therefore really large compared to other protein affinity tags
Description Isotype or positive controls by peptides, antibodies and deactivated samples.The kilo Daltons subunit weight of Recombinant purified Myc-tag -Protein (~9 ) control +ve control compared to your protein ladder can be shifted a little due to electrophoresis effects. 1 kDa = 1000 g/mol protei タグは数アミノ酸から数十アミノ酸の比較的短いポリペプチド鎖からなり、抗体で認識されるタグをエピトープタグと呼びます。遺伝子工学的手法により目的のタンパク質に目印(タグ)をつけて生体内に発現させることで、他の類似のタンパク質と区別することができます Anti-Myc/c-Myc Antibody (9E10) is a mouse monoclonal IgG 1 κ Myc/c-Myc antibody, cited in 9,066 publications, provided at 200 µg/ml; raised against an epitope corresponding to amino acids 408-439 within the C-terminal domain of c-Myc of human origin; This Myc antibody is recommended for detection of c-Myc p67 and c-Myc tagged fusion proteins of mouse, rat, human, monkey, feline and canine. Size Unit Price Quantity; MYC11-C: 100 ul: 195.0 Recombinant purified Myc-tag -Protein (~9 kda) control WB +ve control. Antigen: MYC11-C protein for Western blot +ve control (Cat # MYC11-C) is supplied in SDS-PAGE sample buffer (reduced). Load 10 ul/lane of.
How much would you estimate the the final product of a 122 kDa protein is we detect the protein using a myc fusion tag and get a similar result as you so their size may not reflect on. The Cf-9 sequence predicts a protein of 94 kDa, with 22 glycosylation sites. Using c-myc antibodies, c-myc : Cf-9 protein was detected as a unique band with a molecular size of 160 kDa. The band shifted to approximately 105 kDa after glucosidase treatment, indicating that Cf-9 protein is highly glycosylated Anti-Myc tag Mouse Monoclonal Antibody (100029-MM08), manufactured by Sino Biological is validated in WB,IP. Custom antibody services and bulk production also available. To learn more comprehensive our antibody product information including immunogen, specificity, and more, you can read all details here Like Phage said His mol wt is 155 and if your protein size is 40 kda , the recombinant protein with His tag will be 40,000+930=40930 da. which is just increase of ~1kda . The pET vector that you are inducing alone does not contain your gene, that is why it gives you 15-20 kda (~136-180 amino acids). you need to calculate from the vector sequence what are these amino acids
We fused a Myc-tag in-frame to the 5′-end of def full length cDNA and injected the myc-def mRNA into one while the predicted size for human p53 is 43.7 kDa it runs as a 53 kDa band in an SDS. Flag Size Kda. By Tania Sari | August 30, 2017. 0 Comment. Positive control lysate w00 001 383 protein peptide s flag ged pitx2a flag rabbit polyclonal antibody rebinant dcas9 protein for crispr. Flag Monoclonal Antibody Cusabio. Flio Increases Expression Of Flip Flag Ged 25 4 Kda Was Scientific Diagram Ty1 tag, for instance, 3x Ty1 tag, is preferable for crosslinking ChIP experiments, since FLAG and Myc tags contain lysine, the major target of formaldehyde crosslinking. T7 tag, an 11 amino acid peptide encoded in T7 bacteriophage major capsid protein, exploits the efficient T7 RNA polymerase expression system Plasmid pQCXIH-Myc-YAP from Dr. Kunliang Guan's lab contains the insert YAP and is published in Genes Dev. 2007 Nov 1. 21(21):2747-61. This plasmid is available through Addgene Human c-Myc, also known as MYC proto-oncogene, transcription factor p64, GenBank Accession No. NM_002467, a.a. 2 - 454(end), N-terminal His-tag MW = 52 kDa, expressed in an E. coli expression system
Description 2x Myc-peptide, 2x c-Myc peptide. Sequence EQKLISEEDLEQKLISEEDL The single myc-tag motif EQKLISEEDL corresponds to amino acids 410-19 of the C-terminal domain of human c-myc. Applications Elution of myc-tagged fusion proteins bound to Myc-Trap ® or anti-Myc antibodies.. Note: Optimal dilutions/concentrations should be determined by the use 안티-Myc/c-Myc 항체 9E10 는 마우스 monoclonal IgG 1 κ Myc/c-Myc 항체, 9,056간행물에 인용, 이며 200 µg/ml으로 제공합니다.; epitope corresponding to amino acids 408-439 within the C-terminal domain of c-Myc of human의하고 기원한다; Myc/c-Myc 항체 (9E10)는 WB, IP, IF, IHC(P), FCM and ELISA으로 mouse, rat, human, monkey, feline and canine유래의 c-Myc p67. Two distinct bands of the expected size (approximately 185 and 160 kDa for Cf-9:TAP and Cf-9:myc, and approximately 165 and 145 kDa for Cf-4:TAP and Cf-4:myc, respectively) were detected when using the anti-c-myc antibody (Figure 6a, left), as the protein A moiety in the TAP tag is recognized by any antibody Citrine was fused to the N-terminus while a MYC tag was added to the C-terminus of BioID, BioID2 and (bottom panel). The molecular weight size markers in kDa are indicated at the left of each.
About ReadyTag anti-c-myc. The 9E10 monoclonal antibody reacts with human c-myc, a 62 kDa transcription factor. c-Myc is commonly added to proteins of interest using recombinant DNA technology. The c-myc tag can then be used in many different assays that require recognition by an antibody Plasmids 101: Protein tags. Protein tags are usually smallish peptides incorporated into a translated protein. As depicted in the accompanying cartoon, they have a multitude of uses including (but not limited to) purification, detection, solubilization, localization, or protease protection. Thus far Plasmids 101 has covered GFP and its related.
Myc-OFD1-FL (FL) transfected HEK293 whole cell lysates. Expression of the recombinant proteins was confirmed using antibodies against the myc tag. The predicted sizes of Myc-OFD1-FL (111 kDa) and Myc-OFD1-CTD (100 kDa) are indicated. Two bands are detected at the predicted size of the two OFD1 splice variants in the untransfected sample with Ab2 히스택(His-tag, polyhistidine-tag)은 단백질 내 최소 6개 이상의 히스티딘 (Histidine, His) 잔기(residue)로 구성된 아미노산 모티프로 보통 단백질의 N말단이나 C말단에 있다.이는 또한 헥사히스티딘택(Hexa histidine-tag), 6X히스택(6xHis-tag), 히스6 택(His6 tag)등으로도 알려져 있으며 히스택(His-tag, EMD 바이오사이언스.
Detection of Mouse c‑Myc by Western Blot. Western blot shows lysates of BaF3 mouse pro-B cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse c-Myc Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3696) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017).A specific band was detected for c-Myc at approximately 56 kDa (as indicated) Toggle navigation. +32-(0)1-658-90-45; lieven@gentaur.com; home; about us; Products; contact us; blog; Quality System Manual; Filter Fusion tags relevant for this manuscript can vary greatly in size, from large tags such as the Aequorea victoria green fluorescent protein (GFP) with a size of 26,9 kDa, to small peptide tags including multiple copies of c-myc and HA, each around 1 kDa. GFP tag is an invaluable tool for localizing proteins in both living and fixed cells [5-7. Predicted band size: 49 kDa Observed band size: 57 kDa why is the actual band size different from the predicted? Lanes 1 - 4: Merged signal (red and green). Green - ab32072 observed at 57 kDa. Red - loading control, ab8245 observed at 37 kDa. ab32072 was shown to react with MYC in wild-type HEK-293T cells
Popular tags include the FLAG tag, the hemagglutinin (HA) tag derived from the human influenza virus HA surface protein (6-8), and the Myc tag derived from the human c-Myc oncoprotein . Small tags have the advantage that in many cases they can be fused to the protein of interest without affecting its structure, function, subcellular localization, or stability The maximal size of protein to diffuse through the nuclear pore is larger than 60 kDa Ruiwen Wanga, Michael G. Brattainb,* a Department of Immunology, Roswell Park Cancer Institute, Buffalo, New York 14263, USA b Department of Pharmacology and Therapeutics, Roswell Park Cancer Institute, Elm & Carlton Street, Buffalo, New York 14263, USA. HRP Anti-Myc tag antibody [9E10] (ab62928) at 1/10000 dilution + E. coli Positive Control (Escherichia coli ) Whole Cell Lysate ( ab5395) at 0.1 µg. Performed under reducing conditions. Predicted band size: 41 kDa An approximately 400 kDa membrane-associated complex that contains one molecule of the resistance protein Cf-4. By Jacques Vervoort. The F-Box Protein ACRE189/ACIF1 Regulates Cell Death and Defense Responses Activated during Pathogen Recognition in Tobacco and Tomato. By Owen Rowland Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence
PGC1a antibody Rabbit Polyclonal from Proteintech validated in Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), chImmunoprecipitation (IP),Enzyme-linked Immunosorbent Assay (ELISA) applications. This antibody reacts with human, mouse samples. Cat.No. 20658-1-AP. KD/KO Validated MYCN antibody Rabbit Polyclonal from Proteintech validated in Western Blot (WB), Immunohistochemistry (IHC),Enzyme-linked Immunosorbent Assay (ELISA) applications. This antibody reacts with human, mouse, rat samples. Cat.No. 10159-2-AP. KD/KO Validated Mouse anti c-myc antibody, clone 9E10 detects the p62 c-myc proto-oncogene protein, which is involved in the regulation of the cell cycle and cell growth. C-myc is primarily located to the cell nucleus, but has also been shown to localized to the cytoplasm in several cell lines (Craig et al. 1993).Overexpression of c-myc has been reported in a wide variety of human cancers (Nesbit et al. 1999) All lanes : Biotin Anti-Myc tag antibody [Myc.A7] (ab173829) at 1/1000 dilution Lane 1 : E. coli lysate containing a multi-epitope tagged protein at 0.25 µg Lane 2 : E. coli lysate containing a multi-epitope tagged protein at 0.125 µg Lane 3 : E. coli lysate containing a multi-epitope tagged protein at 0.0599999986588955 µg Lane 4 : E. coli lysate containing a multi-epitope tagged protein.
Affinity tag Sequence or size (kDa) Affinity matrix Elution strategy the Myc tag and the V5 tag, to name a few. Similar to the His tag, these small epitope tags generally do not interfere with protein folding or function. Among them, Myc is mainly used for detection rather than purification [22] Protein tags Large-sized proteins when tagged with a protein of interest result in fusion protein. Some epitope tags, such as myc, are often used in tandem to mCherry Fluorescent tag 28 kDa Due to its improved brightness, superior photostability, and extremel Multi Fusion-Tagged recombinant Protein 52-Kda containing 16-tags (T-7, HSV, C-myc, VSV-G, Glu-Glu Homepage » Products » Multi Fusion Tagged Recombinant Protein Kda Containing Tags T Hsv C Myc Vsv G Glu Glu V E Tag Flag S Tag Ha Kt E Au Au Xhis Tags For Elisa Western. id: MFPM52-C. Size. 100 ul. 286.00. Tag : MYC/DDK: Molecular Mass : 86.2 kDa: Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining: Concentration : >50 ug/mL as determined by microplate BCA method: Storage Buffer : 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol Human recombinant purified His-tag c-myc protein (~65 kda) control ELISA Kits, Antibodies, Pathway Inhibitors, Antibody Arrays Human recombinant purified His-tag c-myc protein (~65 kda) control - Jomar Life Researc
Unlike results obtained with the anti c-myc tag antibody, sc-135063 antibody did not produce a ladder of immunoreactive bands of higher MW. Instead, it generated bands of higher MW (50 and 75 kDa respectively) that had similar intensity regardless of the amount of pcDNA 3.1/c-myc-MasR plasmid used for transfection Size 100 µL Species Reactivity Human Host/Isotype Rabbit / IgG Class Polyclonal Type Antibody (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a FGFR1OP2 Polyclonal Antibody (Product # PA5-58500). For Research Use Only. Not for use in diagnostic procedures The overexpressed (6-myc-SLMAP3) isoform is detected by both anti-SLMAP and anti-Myc antibodies at 110 kDa further discriminating the Tg mice from Wt. No differences in expression of the short SLMAP isoforms, SLMAP1 (~35 kDa) and SLMAP2 (~43 kDa), or endogenous SLMAP3 (~91 kDa) were observed between Wt and Tg mice (Fig 1D and 1E) Size ~150 kDa unconjugated (IgG) ~150 kDa unconjugated (IgG) Only ~15 kDa unconjugated: Binding Affinity: and target a variety of common tags, Myc, GST, & MBP. Nano-Traps are also available for DNA methyltransferase (Dnmt-1), p53, and PARP-1 for immunoprecipitation of untagged, endogenously expressed protein Affinity tags are very useful tools for protein purification. Fused to the protein of interest, they streamline the purification process by binding to a tag-specific resin. Obviously, tag selection is an important step as the purification tag can affect expression level, solubility, facilitate correct folding, protect from proteolysis, and re-direct proteins to a cellular compartment
Two major proteins, 60 and 37 kDa in size, were detected in infected cells with an antibody directed against the carboxy-terminal HA epitope tag of RCAS-Shh (Figure 1A). These two proteins correspond to full-length Shh and a carboxy-terminal fragment resulting from Shh autoproteolysis [ 27 ] As its second name suggests the tag consists of an amino acid sequence DYKDDDDK. (D=Aspartic acid; K=Lysine; Y=Tyrosine). That brings the total size of the tag to 1012.9 dalton or roughly 1 kDa(1). The Flag-tag can be added either to the N-terminus or the C-terminus of a protein(1), respectively Recombinant human WAS protein, fused to MYC/DDK-tag at C-terminal, was expressed in HEK293. Description : The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton Sumoylation: Sumoylation of the target protein can cause a ~15-17 kDa increase of the observed MW due to the covalently attached SUMO protein. Ubiquitination: Addition of a single ubiquitin (8.5kDa), or a chain of ubiquitin proteins, will increase the target protein's observed MW. Glycosylation: Glycosylation will increase the size of the.
Recombinant FK506 Binding Protein 11, 19 KDa (FKBP11) Protein (Fc Tag). Species: Human. Source: Human Cells. Order product ABIN2005830 Basal subtype breast cancers have a particularly poor prognosis, with high invasiveness and resistance to most targeted therapies. TGFβ and MYC drive central features of basal breast cancer: TGFβ is an autocrine and paracrine signaling factor that drives cell invasion and metastasis, and MYC is a central regulator of cellular proliferation that is upregulated in many cancer types Protein-Tag. Als Protein-Tag, Affinitäts -Tag oder Epitop -Tag (engl. tag für ‚Markierung', ‚Schildchen' oder ‚Etikett') werden in der Biochemie verschiedene, meist kurze Aminosäuresequenzen bezeichnet, mit deren Hilfe Proteine markiert werden können. Die markierten Proteine gehören zu den Fusionsproteinen Broad range protein ladders are available from several suppliers for protein gel electrophoresis and related applications. Protein standards are premixed purified proteins of known molecular weights. This set of proteins contain a wide range of sizes, generally under 20 kDa to over 180 kDa
Recombinant MAX protein, His-tag was expressed in E.coli cells as the full length protein (accession number NP_660087.1) with an N-terminal 6×His tag. The molecular weight of this protein is 20.7 kDa Summaries. Gene Snapshot. early girl ( elgi) encodes a myosin binding protein involved in the regulation of meiosis and Fat signaling pathway. [Date last reviewed: 2019-06-27] Automated Description (Alliance of Genome Resources) Exhibits myosin binding activity and ubiquitin protein ligase activity Mouse anti V5-Tag, clone SV5-Pk1 recognizes the sequence, IPNPLLGLD, present on the P/V proteins of the paramyxovirus, SV5 (Dunn et al.1999).Clone SV5-Pk1 is used to detect recombinant proteins, some of which include transmembrane and secreted proteins, that have labeled with tags containing this sequence (Randall et al.1993 and Zhao et al. 2005) Anti-c-myc MicroBeads Cells were transfected with a vector encoding c-myc-BDCA-2 and labeled with 35S-methionine. The recombinant c-myc-BDCA-2 protein was purified with μMACS Anti-c-myc MicroBeads. Left lane: control purification with Anti-HA MicroBeads. The figure shows an autoradiogram after SDS-PAGE. HA-BDCA-2 Positive cells 3 4 1% 10% Anti-H A western blot against the C-terminal myc tag of SO revealed a similar processing of both, the WT and the Moco-deficient SO-R367H-K380R variant, detected at the same apparent size of ∼53 kDa, which was clearly below the size of the full-length, non-processed SO
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c-myc RNA, and the resulting 35S-labeled proteins were immuno-precipitated under low-stringency detergent conditions that are knownto preserve the Myc-Maxheterodimer. kd, kDa. EEOPRFQSA 1 15 28 Max 70 St M dMax 2740 Genetics: Billaud et al. Downloaded at Microsoft Corporation on July 28, 202 Fis1p is a 17-kDa transmembrane protein of the outer Untagged hFis1 (hFis1-UT), hFis1 fused to green fluorescent protein (GFP), or the Myc epitope tag (hFis1-GFP, hFis1-Myc, GFP-hFis1, Myc-hFis1), and the C The Fis1-specific antibody detected a single band of the expected size (17 kDa) in the peroxisomal. Das HA-Tag ist ein Protein-Tag, der bei der Erzeugung und Charakterisierung von rekombinanten Proteinen verwendet wird.. Eigenschaften. Das HA-Tag basiert auf einem Teil des Hämagglutinins (Position 98-106) und besitzt die Aminosäuresequenz Y PYD VPDYA. Es wurde 1988 von J. Field und Kollegen veröffentlicht und ist eines der älteren Protein-Tags DATA IMAGES. GTX37695 IHC-P Image. Filaggrin antibody detects Filaggrin protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. Filaggrin stained by Filaggrin antibody (GTX37695) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min. Show More +